The Cell Culture Laboratory of the Center for Translational Medicine is a modern, specialized
research facility that plays a key role in the advancement of regenerative and translational medicine.
A particularly important area of the laboratory’s activities is the culture of stem cells,
which form the foundation of numerous scientific and therapeutic projects. The laboratory has developed and implemented
standardized protocols for the isolation and culture of primary stem cells derived from bone marrow
and subcutaneous adipose tissue, as well as for their banking
to enable long-term storage and use in research projects.
The banked stem cells, along with immortalized cell lines, represent unique
and strategic resources for studies focused on developing innovative treatment methods. The laboratory also offers
the possibility of establishing new primary cell lines for individual projects and collaborative
research initiatives.
Cells cultured in the laboratory are used in a variety of applications, including testing
new drugs, developing therapeutic strategies in disease models, and analyzing tissue repair
and regeneration processes. A significant aspect of the laboratory's offer also includes cytotoxicity
assessment of substances using the MTT assay under GLP standards, which allows for precise evaluation of the impact
of tested compounds on cell viability.
A key feature of the laboratory is the provision of optimal and fully controlled
environmental conditions for cell culture. CO₂ incubators allow precise regulation
of temperature, humidity, and gas atmosphere, creating ideal conditions for cell growth and differentiation.
komórek.
Sterility of laboratory processes is ensured by laminar flow hoods, which allow safe
and aseptic handling of biological material. These hoods are essential for routine procedures
These hoods are essential for routine procedures
such as cell passaging, preparation of culture media, and transferring
biological material for further analyses.
An important element of the laboratory is also the inverted fluorescence microscope,
which enables real- time monitoring of cell cultures. This allows not only for the assessment of cell condition and morphology,
but also for advanced fluorescence-based analyses, such as tracking
specific protein expression and evaluating biological processes occurring in cells.
The Cell Culture Laboratory is open to scientific and commercial cooperation and
offers the possibility to conduct experiments
requiring a high level of control and ensuring
the safety and reproducibility of results.
Collaboration Opportunities

• Culture and banking of stem cells and primary cell lines derived from bone marrow and subcutaneous
  adipose tissue,
• Cultivation of immortalized and primary cell lines,
• Establishment of new primary cell lines for research projects,
• Comprehensive cell culture in sterile conditions,
• In vitro studies on cytotoxicity (including MTT assay), cell proliferation, and migration,
• Real-time monitoring of cell cultures using brightfield and
  and fluorescence microscopy,
• Preparation of biological material for further molecular and biochemical analyses,
• Use of cell cultures in translational research projects and testing
  of new bioactive compounds.

For inquiries and collaboration offers, please contact:

Head of the Cell Culture Laboratory
MSc Joanna Brzezicka joanna_brzezicka@sggw.edu.pl; tel: + 48 22 593 38 24
CMT Secretariat
cmt@sggw.edu.pl; tel: +48 22 593 38 40