The Histology & Microscopy Laboratory of the Center for Translational Medicine specializes in the comprehensive preparation of tissues for microscopic analysis. The facility is equipped with fully automated systems for processing both formalin-fixed and frozen materials, ensuring high reproducibility of results and minimizing technical variability.

The preparation process covers all necessary steps — from tissue collection, fixation, and dehydration in an automated tissue processor, paraffin embedding, precision sectioning with a rotary microtome, to staining on microscope slides.

The scope of analysis includes the preparation of specimens for classical histopathological evaluation using routine staining methods (hematoxylin and eosin, PAS, Masson’s trichrome) for light microscopy, as well as advanced analytical techniques such as immunohistochemistry and immunofluorescence, followed by imaging via fluorescence microscopy, confocal microscopy, or tissue cytometry.

The prepared histological samples are a key component of pathomorphological assessment, complementing the macroscopic evaluation of organs and tissues obtained during experiments. These specimens allow for detailed recognition of structural changes at the cellular and subcellular levels, which is crucial in toxicological studies, the assessment of the extent and nature of organ damage, analysis of active substance distribution, and in pharmacokinetic and bioavailability studies of new drugs. Microscopic preparations are also utilized to analyze tissue regeneration processes, monitor immune responses, and assess the effectiveness of experimental therapies.

Thanks to advanced equipment and fully automated procedures, it is possible to quickly prepare large series of slides with excellent reproducibility, which is particularly important in projects evaluating the safety, efficacy, and toxicity of tested substances.

Histology Laboratory Equipment::

• Leica ST5020 – automated stainer for routine histological staining,
• • Leica BOND RX – automated system for immunohistochemistry and immunofluorescence staining,
• • Leica RM 2255 – automatic rotary microtome for precise sectioning,
• Leica CM 1860UV – cryostat for preparing frozen tissue sections,
• Leica TP 1020 – automatic tissue processor for dehydration and embedding,
• Leica EG 1150 – embedding station for paraffin samples.

Microscopes: 

• Leica confocal microscope with white laser,,
• Olympus confocal microscope with spinning disk, tissue cytometry, and AI system for cellular structure recognition, counting, and mapping,, 
• Zeiss tissue cytometer for recognition, counting, and mapping of structures in classical and fluorescence histological preparations,,
• Leica two-photon microscope for in vitro and in vivo live imaging studies. in vitro i in vivo.

Collaboration Opportunities 

The Histology Laboratory offers a wide range of services and collaboration formats for scientific research and experimental projects, including:

• Comprehensive preparation of tissue samples for microscopic analysis (fixation, dehydration, embedding, sectioning, staining),
• Routine histological staining (H&E, PAS, Masson’s trichrome, and others) for morphological assessment,
• Advanced immunohistochemical and immunofluorescent staining for the detection of specific antigens and cellular structures,
• Preparation of specimens for light, fluorescence, and confocal microscopy, as well as tissue cytometry,,
• Determination of proliferation (mitotic index) and apoptosis markers in tissues,
• Histometric analysis of tissues (area measurement, cell counting, structure density),
• Analysis of the distribution of biologically active substances in tissues(biodistribution studies),
• Support in interpreting histopathological results and preparing scientific documentation for publication,,
• Evaluation of tissue regeneration in experimental models,
• Monitoring immune responses at the tissue level.

The Histology Laboratory remains open for research collaborations, including customized projects that involve the development of new staining methods and validation of immunohistochemical and immunofluorescence protocols.

For inquiries and collaboration offers, please contact:


• Head of the Histology Laboratory:

Dr. Karolina Ferenc karolina_ferenc@sggw.edu.pl

• CMT Secretariat 

cmt@sggw.edu.pltel: +48 22 593 38 40

A set for fixation and preparation of microscopic specimens from preserved tissues

 Automated slide staining systems;

Advanced confocal microscopy systems, including confocal microscopy with a white laser, tissue cytometry, and two-photon microscopy for the analysis of microscopic specimens and live in vitro and in vivo imaging; in vitro i in-vivo.

 Selected scientific publications in collaboration with the Histology and Microscopy Laboratory.: 

Ferenc Karolina, Marcinkowski Michał, Olszewski Jarosław, Kowalczyk Paweł, Pilžys Tomaš, Garbicz Damian, Dib Naser, Matyba Piotr, Gajewski Zdzisław, Zabielski Romuald: The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs, Scientific Reports, Nature Publishing Group, vol. 14, nr 1, 2024, 10235, s. 1-18, DOI:10.1038/s41598-024-60022-9

Ferenc Karolina, Marcinkowski Michał, Olszewski Jarosław, Kowalczyk Paweł, Pilžys Tomaš, Garbicz Damian, Dib Naser, Matyba Piotr, Gajewski Zdzisław, Zabielski Romuald: The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs, Scientific Reports, Nature Publishing Group, vol. 14, nr 1, 2024, 10235, s. 1-18, DOI:10.1038/s41598-024-60022-9

Konieczka Paweł, Ferenc Karolina, Jørgensen Jens N., Hansen Lea H.B., Zabielski Romuald, Olszewski Jarosław, Gajewski Zdzisław, Mazur-Kuśnirek Magdalena, Szkopek Dominika, Szyryńska Natalia: Feeding Bacillus- based probiotics to gestating and lactating sows is an efficient method for improving immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning, Animal Nutrition, KeAi Communications Co, vol. 13, 2023, s. 361- 372, DOI:10.1016/j.aninu.2023.03.003,