The Molecular Biology Laboratory of the Center for Translational Medicine is a state-of-the-art research facility
designed to support comprehensive analyses in molecular biology, genetics, and cell biology
The laboratory is dedicated to conducting scientific and translational projects
where precise analysis of biological material at the DNA, RNA,
and protein levels is of key importance.
The scope of the Laboratory’s capabilities includes a wide range of studies — from the isolation and
assessment of the quality and quantity of genetic material, through gene expression analysis, to precise protein quantification
and cell phenotyping. This enables both the identification of molecular changes in response to
various factors and the evaluation of outcomes in experimental therapies.
The Laboratory routinely performs gene expression analysis using real-time PCR and
and qPCR techniques, assesses the quality and quantity of DNA and RNA in biological samples, and conducts
protein expression analysis via immunochemical methods such as ELISA and Western-blot.
blot.
It also offers advanced cytometric analysis, allowing
It also offers advanced cytometric analysis, allowing multiparametric characterization of cells and subpopulations. These analyses include
cell cycle evaluation, detection of specific markers, and analysis of intracellular
and surface proteins.
Thanks to its advanced equipment and highly qualified staff, the Molecular Biology Laboratory
provides an excellent environment for scientific research, the development of new diagnostic methods,
and the support of translational medicine projects.
Equipment of the Molecular Biology Laboratory

• • Protein imaging systems (ChemiDoc, Odyssey CLX),
• • Agilent 2100 Bioanalyzer for DNA, RNA, and protein analysis,
• • Thermocycler for classical PCR,
• • Real-time PCR system (QuantStudio 6 FLEX),
• • Automated cell culture monitoring system (JuliBr),
• • Laminar flow hood for sterile work,
• • Ultracentrifuge for isolation and purification of cellular components,
• • Flow cytometer for advanced cell analysis,
• • Multi-mode plate reader for rapid analysis of biological samples.

Collaboration Opportunities
The Molecular Biology Laboratory offers various scientific and
research project options, providing support in the following areas:

• • Qualitative and quantitative analysis of genetic material and proteins,
• • Gene and protein expression studies and evaluation of cellular process dynamics,
• • Multiparametric cell characterization using flow cytometry,
• • Comprehensive data analysis and preparation of scientific publications.

For inquiries and collaboration offers, please contact:

Head of the CMT Laboratory Team
Dr. Eng. Ewa Sady-Makarewicz ewa_sady@sggw.edu.pl; tel: + 48 22 593 38 24
• CMT Secretariat:
cmt@sggw.edu.pl; tel: +48 22 593 38 40